Anti-Connexin 43/GJA1 Antibody Picoband™

Connexin 43/GJA1 antibody

Boster Bio Anti-Connexin 43/GJA1 Antibody Picoband™ catalog # A00599. Tested in ELISA, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 14 publication(s).

Product Info Summary

SKU: A00599
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, IF, IHC, ICC, WB

Product Name

Anti-Connexin 43/GJA1 Antibody Picoband™

View all Connexin 43/GJA1 Antibodies

SKU/Catalog Number

A00599

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Connexin 43/GJA1 Antibody Picoband™ catalog # A00599. Tested in ELISA, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Connexin 43/GJA1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00599)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human Connexin 43/GJA1 recombinant protein (Position: D3-R362).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00599 is reactive to GJA1 in Human, Mouse, Rat

Applications

A00599 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

43 kDa

Calculated molecular weight

43.008kDa

Background of Connexin 43/GJA1

Connexin 43 (Cx43), also called GAP Junction Protein, alpha-1 (GJA1). Connexin 43 is a member of the connexin gene family which abundantly expressed in the heart and liver and was mapped to 6q21-q23.2. Connexin43, the major protein of gap junctions in the heart, is targeted by several protein kinases that regulate myocardial cell-cell coupling. Mutations in the connexin43 gap-junction gene, which lead to abnormally regulated cell-cell communication, are associated with visceroatrial heterotaxia. Cx43 must also play a critical role in the physiology of hearing, presumably by participating in the recycling of potassium to the cochlear endolymph.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Direct ELISA, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For GJA1 (Source: Uniprot.org, NCBI)

Gene Name

GJA1

Full Name

Gap junction alpha-1 protein

Weight

43.008kDa

Superfamily

connexin family

Alternative Names

Connexin 43; connexin-43; CX43; CX43gap junction protein, alpha-like; DFNB38; Gap junction 43 kDa heart protein; gap junction alpha-1 protein; gap junction protein, alpha 1, 43kDa (connexin 43); gap junction protein, alpha 1, 43kDa; GJA1; GJAL; HSS; ODD; ODDD; ODOD; SDTY3 GJA1 AVSD3, CMDR, CX43, EKVP, EKVP3, GJAL, HLHS1, HSS, ODDD, PPKCA gap junction protein alpha 1 gap junction alpha-1 protein|connexin-43|gap junction 43 kDa heart protein|gap junction protein, alpha 1, 43kDa

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on GJA1, check out the GJA1 Infographic

GJA1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for GJA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

A00599 has been cited in 14 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Recombinant human brain natriuretic peptide therapy combined with bone mesenchymal stem cell transplantation for treating heart failure in rats

Expression of Cx43 and Pax3 proteins in the human placental villi and decidua during early pregnancy

Engineered myocardial tissues constructed in vivo using cardiomyocyte-like cells derived from bone marrow mesenchymal stem cells in rats

Fibrosis of Collagen I and Remodeling of Connexin 43 in Atrial Myocardium of Patients with Atrial Fibrillation

Apocynin and raisanberine alleviate intermittent hypoxia induced abnormal StAR and 3β-HSD and low testosterone by suppressing endoplasmic reticulum stress and activated p66Shc in rat testes

Mitochondrial Uncoupling Protein 2 in human cumulus cells is associated with regulating autophagy and apoptosis, maintaining gap junction integrity and progesterone synthesis

AQP4 KO exacerbating renal dysfunction is mediated by endoplasmic reticulum stress and p66Shc and is attenuated by apocynin and endothelin antagonist CPU0213

Ectoderm mesenchymal stem cells promote differentiation and maturation of oligodendrocyte precursor cells

Chemical and physical stimuli induce cardiomyocyte differentiation from stem cells

Differential expression of connexin 43 in human autoimmune thyroid disease

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5 Customer Q&As for Anti-Connexin 43/GJA1 Antibody Picoband™

Question

My boss were well pleased with the WB result of your anti-Connexin 43/GJA1 antibody. However we have observed positive staining in pigmented layer of retina cell membrane using this antibody. Is that expected? Could you tell me where is GJA1 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-10-01

Answer

From literature, pigmented layer of retina does express GJA1. Generally GJA1 expresses in cell membrane. Regarding which tissues have GJA1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Cerebellum, Pubmed ID: 14702039
Heart muscle, Pubmed ID: 1696265

Boster Scientific Support

Answered: 2019-10-01

Question

you antibody using your anti-Connexin 43/GJA1 antibody for epididymis development studies. Has this antibody been tested with western blotting on rat brain tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-07-25

Answer

We appreciate your inquiry. This A00599 anti-Connexin 43/GJA1 antibody is validated on rat brain tissue, mouse brain. It is guaranteed to work for ELISA, IF, IHC-P, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-07-25

Question

We are currently using anti-Connexin 43/GJA1 antibody A00599 for human tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on pig tissues as well?

Verified Customer

Verified customer

Asked: 2018-10-26

Answer

The anti-Connexin 43/GJA1 antibody (A00599) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-10-26

Question

We have observed staining in human pigmented layer of retina. What should we do? Is anti-Connexin 43/GJA1 antibody supposed to stain pigmented layer of retina positively?

Verified Customer

Verified customer

Asked: 2018-02-28

Answer

Based on literature pigmented layer of retina does express GJA1. Based on Uniprot.org, GJA1 is expressed in pigmented layer of retina, heart muscle, cerebellum, brain, among other tissues. Regarding which tissues have GJA1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Cerebellum, Pubmed ID: 14702039
Heart muscle, Pubmed ID: 1696265

Boster Scientific Support

Answered: 2018-02-28

Question

We ordered your anti-Connexin 43/GJA1 antibody for IHC-P on brain a few months ago. I am using mouse, and We are going to use the antibody for ICC next. We want examining brain as well as heart muscle in our next experiment. Could you please give me some suggestion on which antibody would work the best for ICC?

Verified Customer

Verified customer

Asked: 2017-07-28

Answer

I looked at the website and datasheets of our anti-Connexin 43/GJA1 antibody and I see that A00599 has been validated on mouse in both IHC-P and ICC. Thus A00599 should work for your application. Our Boster satisfaction guarantee will cover this product for ICC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for ICC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2017-07-28

Order DetailsPrice
A00599

100μg

$370
A00599-10ug

10μg sample (liquid)

$99
A00599-Biotin

100 μg Biotin conjugated

$570
A00599-Cy3

100 μg Cy3 conjugated

$570
A00599-Dylight488

100 μg Dylight488 conjugated

$570
A00599-Dylight550

100 μg Dylight550 conjugated

$570
A00599-Dylight594

100 μg Dylight594 conjugated

$570
A00599-FITC

100 μg FITC conjugated

$570
A00599-HRP

100 μg HRP conjugated

$570
A00599-APC

100 μg APC conjugated

$670
A00599-PE

100 μg PE conjugated

$670
A00599-carrier-free

Carrier Free

$370

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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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